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Total RNA yieldwas quantified by spectrophotometric analysisusing 1 optical density (OD) at 260 nm equals40 ?g RNA per ml.
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Some investigators contend that the basementmembrane includes not only the basal lamina but alsoa secondary layer of small-unit fibrils of type III collagen(reticular fibers) that forms the reticular lamina. In response to mitogen signaling AKT phosphorylation of Mdm2triggers nuclear localization . It is speculated that the onset of ATL is influenced by the decrease of viral loadlevel.
The N-terminal region of p53 is a disordered, natively unfoldedregion  containing the acidic TAD (transactivation domain), which is a bindingsite for some of the p53 interacting proteins such as the transcriptional machineryproteins , and MDM2 .
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These immunoassays detect invitro interferon-gamma secreted by peripheral blood mononuclear cells in response tospecific antigens of M.
 reported in a series of 40 episodes of TKA PJI that 77%of the patients had either early postoperative or late hematogenous infection.
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